Mass spectrometry of proteins and their modifications in single cells: an avenue to revolutionize biomedicine

Single-cell proteomics and single-cell phosphoproteomics are cutting-edge technologies to study proteins and their phosphorylation sites within individual cells. Proteins are essential to a wide range of cellular processes and functions, such as regulating gene expression, catalyzing biochemical reactions, providing structural support, and transporting molecules. Their function and activity can be modulated by phosphorylation sites and the study of this enzymatic modification is key for understanding the proteome in action in individual cells. Recent advancements in mass spectrometry and sample processing techniques have made single-cell proteomics possible. We will further develop these techniques to enable global analysis of phosphorylation sites in single-cells to advance our understanding of cellular processes, disease mechanisms, opening up new avenues for biological and clinical research. The results will hence greatly aid the understanding of human cell biology and disease. _x000D_

Overview

Grant agreement NNF24OC0087868