Optical pooled genetic screening facility
Cells are the fundamental units of life, and imbalances in their organization and function underlie human disease. Genetic screening is a powerful tool for linking genes with cellular phenotype, and the advent of CRISPR-based genome editing has enabled screens with tremendous precision at massive scale. Genetic screening has previously been restricted to simple readouts hampering its use for studying complex biological processes. A recently developed technique termed optical pooled screening overcomes this limitation by coupling microscopy-based phenotyping and genotyping. This technology requires specialized equipment and knowledge, making it inaccessible to most researchers. To overcome this barrier, we will establish the first open-access facility for optical pooled screening, providing access to the required equipment and supporting researchers at all steps of the procedure, making this groundbreaking method broadly available and thereby accelerating biomedical research in Denmark.
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Imported from OpenAIRE | Grant NNF24OC0087805.
Open sourceOverview
Grant agreement NNF24OC0087805
Open sourceCells are the fundamental units of life, and imbalances in their organization and function underlie human disease. Genetic screening is a powerful tool for linking genes with cellular phenotype, and the advent of CRISPR-based genome editing has enabled screens with tremendous precision at massive scale. Genetic screening has previously been restricted to simple readouts hampering its use for studying complex biological processes. A recently developed technique termed optical pooled screening overcomes this limitation by coupling microscopy-based phenotyping and genotyping. This technology requires specialized equipment and knowledge, making it inaccessible to most researchers. To overcome this barrier, we will establish the first open-access facility for optical pooled screening, providing access to the required equipment and supporting researchers at all steps of the procedure, making this groundbreaking method broadly available and thereby accelerating biomedical research in Denmark.